Transcriptome profiling of pyrethroid resistant and susceptible mosquitoes in the malaria vector, Anopheles sinensis
Abstract: Anopheles sinensis is a major malaria vector in China and other Southeast Asian countries, and it is becoming increasingly resistant to the insecticides used for agriculture, net impregnation, and indoor residual spray.
Results: The 454 GS FLX transcriptome sequencing yielded a total of 624,559 reads (average length of 290 bp) with the pooled An. sinensis mosquitoes across various development stages. The de novo assembly generated 33,411 contigs with average length of 493 bp. A total of 8,057 ESTs were generated with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation. A total of 2,131 ESTs were differentially expressed between deltamethrin resistant and susceptible mosquitoes collected from the same field site in Jiangsu, China.
Keywords: Transcriptome, Expressed sequence tag, Pyrethroid resistance, Gene expression, Anopheles sinensis
   1. Rueda LM, Zhao TY, Ma YJ, Gao Q, Zhu GD, Khuntirat B, Sattabongkot J,Wilkerson RC: Updated distribution records of the Anopheles (Anopheles)hyrcanus species-group (Diptera: Culicidae) in China. Zootaxa 2007,1407:43–55.
   2. Zhou SS, Huang F, Wang JJ, Zhang SS, Su YP, Tang LH: Geographical,meteorological and vectorial factors related to malaria re-emergence inHuang-Huai River of central China. Malar J 2010, 9:337.
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